Product Name :
CTCF Recombinant Rabbit Monoclonal Antibody [JM10-61]
Predicted band size :
83 kDa
Observed band size :
130 kDa
Synonyms:
11 zinc finger protein antibody 11 zinc finger transcriptional repressor antibody 11-zinc finger protein antibody CCCTC binding factor (zinc finger protein) antibody CCCTC binding factor antibody CCCTC-binding factor antibody Ctcf antibody CTCF_HUMAN antibody CTCFL paralog antibody MRD21 antibody 展开
Function :
CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes.{{Eribulin} MedChemExpress|{Eribulin} Cytoskeleton|{Eribulin} Biological Activity|{Eribulin} Formula|{Eribulin} custom synthesis|{Eribulin} Autophagy} The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2 class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development. CTCF is ubiquitously expressed and localized to the nucleus.{{Doxepin} MedChemExpress|{Doxepin} Immunology/Inflammation|{Doxepin} Biological Activity|{Doxepin} In Vivo|{Doxepin} custom synthesis|{Doxepin} Epigenetic Reader Domain} During terminal differentiation, CTCF is negatively regulated by differential phosphorylation and also by decreases in CTCF mRNA and protein expression.PMID:24670464
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within Human CTCF aa 685-727 / 727.
Species reactivity:
Human, Mouse, Rat, Zebrafish
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, CUT&Tag-seq, ChIP
Coupling :
unconjugated
Clone number:
JM10-61
RRID:
AB_3070444
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 83 kDa
Positive control :
MCF-7 cell lysate, 293T cell lysate, zebrafish tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, HeLa, MCF-7, NIH/3T3, human liver tissue, human kidney tissue, mouse colon tissue, human endometrium tissue, rat colon tissue, rat kidney tissue.