Product Name :
Anti-V5 tag, Mouse IgG2b antibody
Applications:
WB,ICC/IF,ELISA,IP,Flow Cyt
Reactivity :
V5 tag
Conjugate:
Unconjugated
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-V5 tag, Mouse IgG2b antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, Mouse IgG2b antibody is monovalent, recombinant single domain antibody fused to mouse IgG2b Fc.{{51-21-8} MedChemExpress|{51-21-8} Biological Activity|{51-21-8} In Vivo|{51-21-8} manufacturer} Based on western blot and ELISA, Anti-V5 tag, Mouse IgG2b antibody reacts with the V5 tag sequence(GKPIPNPLLGLDST) selectively, no reactivity with other proteins. | Immunogen: V5 tag fusion protein | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to Mouse IgG2b Fc(mutation) | Conjugate: Unconjugated | Specificity: V5 tag sequence(GKPIPNPLLGLDST) | Cross-Reactivity: Highly selective for V5 tag sequence | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C | Background:The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family.{{1264737-26-9} web|{1264737-26-9} Purity & Documentation|{1264737-26-9} References|{1264737-26-9} supplier} This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-V5 tag, Mouse IgG2b antibody is designed for detecting V5 tag fusion protein specifically.PMID:30222985 Anti-V5 tag, Mouse IgG2b antibody is monovalent, recombinant single domain antibody fused to mouse IgG2b Fc. Based on western blot and ELISA, Anti-V5 tag, Mouse IgG2b antibody reacts with the V5 tag sequence(GKPIPNPLLGLDST) selectively, no reactivity with other proteins.
Immunogen:
V5 tag fusion protein
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to Mouse IgG2b Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Function:
WB: 1:10,000-1:50,000ELISA: 1:10,000-1:50,000ICC/IF: 1:200-1:1000IP: 1-2ug/sampleFlow Cyt：1µg for 106 cellsDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.