Product Name :
CDKN1A/P21 Rabbit Polyclonal Antibody
Predicted band size :
18 kDa
Observed band size :
21 kDa
Synonyms:
CAP20 antibody CDK-interacting protein 1 antibody CDKI antibody CDKN1 antibody Cdkn1a antibody CDN1A_HUMAN antibody CIP1 antibody Cyclin Dependent Kinase Inhibitor 1A antibody Cyclin-dependent kinase inhibitor 1 antibody Cyclin-dependent kinase inhibitor 1A (P21) antibody 展开
Function :
p21 is a potent cyclin-dependent kinase inhibitor (CKI). The p21 (CIP1/WAF1) protein binds to and inhibits the activity of cyclin-CDK2, -CDK1, and -CDK4/6 complexes, and thus functions as a regulator of cell cycle progression at G1 and S phase. p21 interacts with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. However p21 may inhibit apoptosis and does not induce cell death on its own. The ability of p21 to inhibit apoptosis in response to replication fork stress has also been reported. Studies of p53 dependent cell cycle arrest in response to DNA damage identified p21 as the primary mediator of downstream cell cycle arrest. p21 is negatively regulated by ubiquitin ligases both over the course of the cell cycle and in response to DNA damage. Cytoplasmic p21 expression can be significantly correlated with lymph node metastasis, distant metastases, advanced TNM stage, depth of invasion and OS.
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
Recombinant protein within human CDKN1A/P21 aa 1-164.
Species reactivity:
Human, Mouse
Verify application:
WB, IHC-P
Coupling :
unconjugated
Clone number:
RRID:
AB_2942080
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.{{Inorganic pyrophosphatase} MedChemExpress|{Inorganic pyrophosphatase} Cell Cycle/DNA Damage|{Inorganic pyrophosphatase} Purity & Documentation|{Inorganic pyrophosphatase} Data Sheet|{Inorganic pyrophosphatase} manufacturer|{Inorganic pyrophosphatase} Autophagy} Aliquot store at -20℃.{{α-Vitamin E} MedChemExpress|{α-Vitamin E} Reactive Oxygen Species|{α-Vitamin E} Purity & Documentation|{α-Vitamin E} In stock|{α-Vitamin E} supplier|{α-Vitamin E} Autophagy} Avoid repeated freeze / thaw cycles.PMID:23664186
Storage buffer solution:
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Immunogen affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 18 kDa
Positive control :
U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate, mouse colon tissue lysate, human esophagus tissue.