Product Name :
CD79a Recombinant Rabbit Monoclonal Antibody [JE43-50]
Predicted band size :
25 kDa
Observed band size :
38 kDa ET7109-33) at 1/100 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-33) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.” style=”width: auto”> Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD79a antibody (ET7109-33) at 1/100 dilution.
Synonyms:
B lymphocyte-specific MB1 protein antibody B-cell antigen receptor complex-associated protein alpha chain antibody CD 79a antibody CD79a antibody CD79a antigen (immunoglobulin-associated alpha) antibody CD79A antigen antibody CD79a molecule, immunoglobulin-associated alpha antibody CD79A_HUMAN antibody Ig alpha antibody Ig-alpha antibody 展开
Function :
Cluster of differentiation CD79A also known as B-cell antigen receptor complex-associated protein alpha chain and MB-1 membrane glycoprotein, is a protein that in humans is encoded by the CD79A gene. The CD79a protein together with the related CD79b protein, forms a dimer associated with membrane-bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR). This occurs in a similar manner to the association of CD3 with the T-cell receptor, and enables the cell to respond to the presence of antigens on its surface. It is associated with agammaglobulinemia-3. CD79a plays multiple and diverse roles in B cell development and function. The CD79a/b heterodimer associates non-covalently with the immunoglobulin heavy chain through its transmembrane region, thus forming the BCR along with the immunoglobulin light chain and the pre-BCR when associated with the surrogate light chain in developing B cells. Association of the CD79a/b heterodimer with the immunoglobulin heavy chain is required for surface expression of the BCR and BCR induced calcium flux and protein tyrosine phosphorylation. Genetic deletion of the transmembrane exon of CD79A results in loss of CD79a protein and a complete block of B cell development at the pro to pre B cell transition. Similarly, humans with homozygous splice variants in CD79A predicted to result in loss of the transmembrane region and a truncated or absent protein display agammaglobulinemia and no peripheral B cells.{{Prodigiosin} MedChemExpress|{Prodigiosin} Apoptosis|{Prodigiosin} Protocol|{Prodigiosin} Description|{Prodigiosin} manufacturer|{Prodigiosin} Epigenetics}
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within Human CD79a aa 50-100 / 226.{{Pyrroloquinoline quinone} MedChemExpress|{Pyrroloquinoline quinone} Endogenous Metabolite|{Pyrroloquinoline quinone} Protocol|{Pyrroloquinoline quinone} References|{Pyrroloquinoline quinone} manufacturer|{Pyrroloquinoline quinone} Epigenetic Reader Domain}
Species reactivity:
Human
Verify application:
WB, IF-Tissue, IHC-P, FC
Coupling :
unconjugated
Clone number:
JE43-50
RRID:
AB_3070921
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.PMID:23415682 Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 25 kDa
Positive control :
Daudi cell lysate, Raji cell lysate, human tonsil tissue, human appendix tissue, human spleen tissue, Daudi, human lymph nodes tissue.