Atmosphere for 40 min then rinsed 3 times for 5 min inside the
Environment for 40 min then rinsed three occasions for 5 min inside the washing solution. A second anti-rabbit Ig antibody coupled to ALEXA 488 was placed on the slides which had been then placed back into a moist environment for 40 min. A final washing (three 5 min) was performed ahead of slide assembly and […]