Day: August 9, 2024

By incubation at 37uC for 24 h. mRNA levels of miR-33a-5P, ABCA1 and ABCG1 were determined employing RT-PCR. U6 or b-actin served as a reference gene. Information are indicates six SD from six experiments. (C and D) Protein levels of ABCA1 and ABCG1 determined by Western blotting. (E and F) Quantification of densitometric values of […]

S (2, five, 7, 13, 17, and 22) had been coupled to an NHS-activated PEGylated lipid and formulated into fluorescent, one hundred nm liposomal nanoparticles displaying a five molar amount of the numerous ligand-lipids or, as a handle, 5 of a PEGylated lipid containing no ligand (`Naked’). Liposome binding to each cell lines, as assessed […]