NM, 0.05 nM and 0.02 nM, respectively. Inside the experiment involving magnetic beads covalently cross-linked
NM, 0.05 nM and 0.02 nM, respectively. Inside the experiment involving magnetic beads covalently cross-linked to tag antibodies, we made use of anti-DYKDDDDK tag antibody magnetic beads (clone IE6, Wako) and MagCapture HP anti-PA tag antibody magnetic beads (clone NZ-1, Wako). HiBit detection assays.The immunoprecipitated samples were diluted 100-fold employing PBS containing 0.01 BSA and […]