Ious concentrations. The cellular luciferase PF-06250112 Protein Tyrosine Kinase/RTK activity was determined using Dual-Glo Luciferase
Ious concentrations. The cellular luciferase PF-06250112 Protein Tyrosine Kinase/RTK activity was determined using Dual-Glo Luciferase Assay System (Promega) and EnVision Multilabel Reader (PerkinElmer). Firefly luciferase activity was normalized for the co-expressed Renilla luciferase activity. All the luciferase assays were run in triplicate, and also the outcomes are shown as implies ?SEM.Cell culture.Quantification and statistical analysis. […]